Sentence examples for multiple integration cassettes from inspiring English sources

To engineer recombinant yeast that stably maintains expression cassettes without an antibiotic resistance gene, we developed new multiple integration cassettes by exploiting the non-transcribed spacer (NTS) of ribosomal DNA (rDNA) in combination with defective selection markers.

The multiple integrated cassettes were stably retained under prolonged nonselective conditions.

Two-step PCR was used to amplify the integration cassettes.

Despite this anomaly, the data reported here suggest that integration of foreign antigen cassettes into multiple loci within a live vector chromosome can be accomplished without further attenuation of the vaccine strain, and that this multiple integration strategy results in superior expression levels of foreign antigens vs. conventional integration into a single locus.

Five transformants showed tandem integration and one multiple integration.

The integration cassettes were digested from the plasmids with BglII/HindIII prior to transfection.

It also highlights the problem of genetic instability of the integration cassettes that might be encountered when cultivating multicopy strains.

The integration cassettes from pGL1229 and pGL1005 were excised by digestion with PacI and PmeI before transfection.

As suggested above, this result could be due to multiple integrations of the expression cassette or to chromosomal position effects.

The resulting integration cassette was integrated into the promoter of cdc25 +.

We observed multiple integrations in most clones.