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Morphological reconstructions were produced manually from multiple image stacks.
However, axons go across multiple image stacks and it is important to make sure that the same axon is given the same name and color in all the stacks it traverses.
Only one mosaic was taken, and from this mosaic multiple image stacks of suspicious regions were acquired.
The region covered by the intracellularly filled neurons was entirely scanned at high resolution by automatically acquiring multiple image stacks with the Arivis Browser (Arivis Multiple Image Tools GmbH, Rostock, Germany).
Quantifying this in a meaningful way is problematic as these 3D-SIM images are all calculated reconstructions from multiple image stacks that, for technical reasons, have to be acquired sequentially (red first then green); the PCM is rapidly moving in these embryos making voxel by voxel comparisons unreliable, particularly in the crowded environment of the centrosome.
Similar(55)
In order to truly determine the incidence of pheomelanotic melanoma lesions among those diagnosed as "amelanotic", this proof-of-concept work will have to be expanded into a broader study: one where multiple CARS image stacks taken from a large patient cohort will be processed and statistically analysed to gain insight that can impact clinical practice.
The image is a maximum-intensity projection of multiple aligned image stacks.
Multiple 3D image stacks with dimensions 1000 × 512 × 256 (A-scans x Pixels x B-scans) were acquired from the chicken retina at wide (~20°) and narrow (~7°) scanning angles.
Cells were monitored for 8 h, and multiple image z-stacks were captured every 5 min.
After recordings, hippocampal slices were fixed in 4% paraformaldehyde in PBS overnight and multiple high resolution image stacks (typically 120×120 µm, 1024×1024 pixels; Δz = 0.75 µm) covering the entire cell, were taken with a Leica Confocal microscope (LCS SP2).
Multiple images were stacked together to build up a three-dimensional reconstruction of the original sample.
Related(20)
multiple module stacks
multiple image streams
multiple image panels
multiple image segmentations
multiple image attacks
multiple image fields
multiple image subjects
multiple image areas
multiple image techniques
multiple image extensions
multiple image scans
multiple image blocks
multiple image sequences
multiple image frames
multiple image lines
multiple image modalities
multiple image data
multiple image z-stacks
multiple image variations
multiple image planes
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