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Multiple image fields were usually necessary for complete coverage of the tissue sections.
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Multiple images from random fields were captured using an Optronics DEI750 CCD camera and Adobe Photoshop software.
Since multiple images of adjacent fields of view were obtained at each site in order to increase the region surveyed, the feature values calculated for each image were grouped by site and then averaged.
Multiple images were acquired from adjacent fields of view at each location on the specimen by translating the probe position approximately 1 mm.
The arithmetic data processing includes dark signal subtraction, dead pixel correction, computation of median from multiple images, averaging of images, and flat field correction.
Focus stacking is a computational technique to extend the Depth of Field (DOF) through combining multiple images taken at various focus distances.
3-D images can be obtained by scanning the array in a direction perpendicular to the 2-D image field and applying suitable image processing to the multiple scanned 2-D images.
Multiple channels containing different samples were observed simultaneously in a single image field (Figure 2B).
However, considering the size of the cells and the image field, as well as the need to acquire multiple images, all formats smaller than 96-wells did not make any sense for our experimental system.
Because the scale of the corneas is much larger than the field of view of the microscope's objective, multiple images were taken using a computer-controlled stage and automatically stitched together in software.
The field of view is ~ 2 cm × 5 cm and multiple images, showing parameters such as X-ray absorption, refraction and scatter width are obtained [32, 33].
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