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With the use of multiple image planes including unusual imaging windows such as the right parasternal, left parasternal, and paraspinal, the thoracic aorta can be imaged in its entirety increasing the accuracy of TTE [6].
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Acquisition of flow vector information at high frame rates is obtained by performing multi-directional transmissions of plane waves; after a single plane wave transmission, multiple image receiving lines are obtained [23].
Image Planes.
Multiple images from each z plane were stitched to form a complete overview image using Zen 2011/2012 software.
Post-processing techniques such as structured illumination [ 1] and HiLo microscopy [ 2] require multiple images to be acquired for each imaging plane.
An image is referred to a frame recorded in the coronal plane (inset of Fig. 1(b)) of the sciatic nerve as opposed to a transverse image (inset of Fig. 1(a)) which is rendered from a z-stack of multiple images recorded in the coronal plane.
A diffraction grating is placed at the image plane, thus generating multiple diffraction orders containing full spatial information about the image.
This required that we take multiple images of the same cell in different planes, often in two different channels (GFP and mCherry).
Analysis of imaging properties of QGPS in terms of point-spread function shows that QGPS can image multiple layers within an object field onto a single image plane.
3D-SIM requires both more image frames per focus plane and also the acquisition of images across multiple focal planes in order to reconstruct a single image plane.
Imaging multiple planes in the z-axis reveals filamentous staining in the cortex, but the microfilaments appear to be randomly oriented (Figure S1).
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