Sentence examples for multiple expression cassettes from inspiring English sources

Moreover, our work provides hints on the design of lentiviral vectors containing multiple expression cassettes with multiple promoters.

We expect that this approach will be useful for the construction of strains carrying multiple expression cassettes for analysis of trypanosomatid pathogenicity mechanisms and overexpression of multi-subunit protein complexes for biochemical and structural studies.

We discuss the considerations of using our system in various plant model systems, the use of single versus multiple expression cassettes, the application of our vectors using various transformation methods and the use of internal fluorescent markers which can assist in signal localization and easy data acquisition in living cells.

Each YAC consists of multiple expression cassettes under the control of inducible methionine dependent promoters.

A recent detailed review describes the methods applied to obtain strains containing multiple expression cassettes and provides a summary of published data showing correlations between copy number and expression levels of intracellular as well as secreted proteins.

The only inconvenience of the CRISPR/dCas9 system may exist in the targeting of multiple genes, when multiple sgRNA expression cassettes must be constructed.

However, to produce multiple gene mutations in plants, resources and methods for the assembly of multiple gRNA expression cassettes are frequently required.

Moreover, the simultaneous production of both Si6A and Si6 by Vec26 exerted higher inhibitory effect on the TRAF6 expression (lane 5, Fig. 3c) than the single siRNA (lanes 3 and 4), supporting that the vectors with multiple siRNA expression cassettes have potential to increase the efficiency of gene silencing by expressing different siRNA targeting distinct regions of a gene.

Although these controls showed that retroviral infection indeed caused non-specific inhibitory effect, the data do not affect our conclusion that the vectors with multiple siRNA expression cassettes have potential to increase the efficiency of gene silencing by expressing different siRNA targeting distinct regions of a gene.

For generating multiplex sgRNA vectors, we assembled multiple sgRNA expression cassettes using the three-step Golden Gate cloning method.

We designed PCR-based procedures to rapidly generate multiple sgRNA expression cassettes, which can be assembled into the binary CRISPR/Cas9 vectors in one round of cloning by Golden Gate ligation or Gibson Assembly.