Sentence examples for multiple expression plasmids from inspiring English sources

However, for the same protein to be produced in both systems multiple expression plasmids are typically used since the elements needed for efficient replication, selection, mRNA transcription, and protein translation for the two systems are different.

The method and reagents described in this paper significantly simplifies the construction of expression plasmids from multiple DNA fragments, including complex gene fusions, chimeric genes and polycistronic constructs.

The multiple NANOG gRNA expression plasmid was constructed by SynGene (http://syngen.tech) as depicted in Fig. 2A.

Each primer sequence contains an outer and inner restriction enzyme site for cloning the HCMV sequences at the multiple cloning site (MCS) of the yeast and mammalian expression plasmids, respectively (Table S1) (Supporting Information).

We believe that identification of a highly efficient 2A peptide and construction of expression plasmids with a 2A gene flanked by multiple cloning sites would facilitate the use of 2A technology for simultaneous expression of multiple genes.

While the bursal DT40 cell line has multiple valuable features for research [16], the transfection efficiency of expression plasmids is usually very low.

PC6.3 cells in six-well plates were transfected with 0.5  μg of FL huntingtin expression plasmids in conjunction with 0.5  μg of the NF- κB reporter plasmid, containing multiple NF- κB sites linked to firefly luciferase gene.

To generate shRNA expression plasmids we first used the Whitehead Institute siRNA selection program to design shRNAs targeting multiple regions of Paupar and Pax6.

Fig. 2 Schematic organization of cocoa gene expression cassettes in each of the expression plasmids.

Furthermore, expression profiling can facilitate the design of optimal expression plasmids for use in specific cancers.

The pES31Hneo and pES33Ezeo expression plasmids have been described previously [23].