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Immediately after cervical dislocation one hind limb of a C57Bl/6 mouse was prepared and the whole BM was flushed out of both, tibia and femur, by use of a 21-gauge needle attached to a 10 ml syringe filled with PBS.

The transgene for the JOE mouse was prepared using the 1.9 kb 'classic' MBP promoter plasmid (pMG2), a gift from Dr Robert Lazzarini Goww et al., 1992).

In brief, a microsomal fraction from the liver of the ICR mouse was prepared according to a previously reported method [ 37].

During a recent work, where a knockin SCA28 mouse was prepared, we developed two assays based on Real-Time PCR using both SYBR Green and specific minor groove binder (MGB) probes to evaluate the copies of NEO using the comparative delta-delta Ct method versus the Rpp30 reference gene.

A single-cell suspension of the spleen from a naïve mouse was prepared in R10S medium (RPMI 1640, 10% HI-FCS [Summit Biotechnology, Fort Collins, CO, USA], 1% nonessential amino acids, 100 mM MEM sodium pyruvate, 2 mM L-glutamine, 100 U/ml penicillin, 1 ml streptomycin, and 50 mM 2-mercaptoethanol) by gently swirling the spleen between frosted glass slides.

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The Ehrlich and fibrosarcoma Sa-37 ascitic tumor cell suspensions, transplanted to the BALB/c mouse, were prepared from the ascitic forms of the tumors.

A single cell suspension of pancreatic tumors of 12 -week -old RIP1-Tag2 mice was prepared by Dispase digestion.

RNA from the brain of affected (approximately three month old) dcr mice was prepared using Trizol reagent (Invitrogen, Carlsbad, CA, USA).

The EDIM virus, pooled from fecal specimens of infected neonatal mice, was prepared as previously described.

Liver chromatin from fasted and re-fed mice was prepared as previously described [ 53].

Genomic DNA from mice was prepared from tail tips using standard procedures (DNeasy Tissue Kit, Qiagen, Hilden, Germany).

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