In a pre-test session, each mouse was placed in the water for 15 min.
After 90 seconds the mouse was placed in its original housing cage.
The mouse was placed in the central platform and individually tested for 5 min.
On day one, the mouse was placed in an opaque box in the center of the maze.
The mouse was placed head upward on top of a wooden rough-surfaced pole (diameter 1 cm, height 70 cm).
Each mouse was placed on a metal mesh and pulled horizontally, and the power of traction when the mouse released the mesh was defined as the muscle strength.
Each mouse was placed in the dark chamber and was allowed to run freely between the two chambers for 5 minutes.
To realize ultra-fast imaging of blood flow in brain vessels, a C57 mouse was placed on an imaging stage positioned in front of the InGaAs camera.
Then, the mouse was placed under the water bag of the imaging system, and ultrasound gel was applied between the head and the water bag for ultrasonic coupling.
A mouse was placed onto a horizontally positioned wire (1.5 mm in diameter and 15 cm in length), and the wire was placed 30 cm above the floor.
The entire anesthetized mouse was placed in the dose calibrator, and the dispensed radioactivity was confirmed.
