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The nude mouse was imaged for in vivo PL at 1 h postinjection using the Maestro fluorescence imaging system (CRi).
Each mouse was imaged sequentially with fDOT and PET.
For the 19 F-MR image, the mouse was imaged with a gradient echo sequence (128 × 128 matrix; 3 cm FOV; 56.0 ms TR; 2.6 ms TE; 20 mm slice thickness; 60° flip angle; 256 NEX; 30 min total scan time).
For movie collection, one mouse was imaged for each condition using the catheterized mice.
The mouse was imaged after four hours of its death in order to avoid the small shifts of the body caused by the development of rigor mortis [7].
An adult 3-month old C57/BL6 male mouse was imaged.
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Furthermore, glioblastoma tumor xenografts in a NOD/SCID mouse were imaged.
In addition, glioblastoma brain tumor xenografts from a NOD/SCID mouse were imaged by MALDI-MS.
Brain sections from a C57BL/6 mouse were imaged using this technique at a resolution of 100 μm.
Vibratome sections (3 5 per mouse) were imaged at 40X with a 2.4-μm optical slice and Cux-1 cells were counted in an 8 bin analysis.
The mice were imaged weekly using IVIS imaging.
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