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Most important, our approach enables repeated visualization and intervention within the same mouse, such that inter-animal variability can be avoided and detection of temporal changes can be optimized.
Our results show also significant divergence between pigs and mouse such that there was no mouse ortholog for porcine GPR41.
Mice were scored for all paws, and scores were summed per mouse such that the clinical grade for a single mouse could range from 0 to a maximum of 16.
Grades from the three readers were averaged for each section, then scores for all gradable sections were averaged for each mouse, such that each mouse had one grade for tibial cartilage and one grade for femoral cartilage.
Training for the challenging beam traversal test and the pole test were done consecutively for each mouse, such that each mouse completed training for the pole before training for the beam in a single session.
Mice were scored individually for each eye, and scores were summed per mouse such that the clinical grade for a single mouse could range from 0 to a maximum of 4. At the indicated times, the eyes and ankles of each animal were collected, fixed in 10%% neutral-buffered formalin and prepared for tissue sectioning as previously reported [ 26, 27].
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Dex treatment alone sedated, but did not anesthetize the mice, such that dynamic PET acquisition was not possible in this treatment group.
Renograms proved to be highly variable in mice, such that we decided to test the FUR quantitative approach, which provided a more sensitive index of the extent of renal failure at day 65.
Deletion of only icl1 impaired persistence of Mtb in mice such that the CFUs isolated from lungs during chronic infections were reduced by approximately 90% [40], [44].
With repeated Hole Board testing grooming increased in both groups, but control mice increased their grooming periods more than mutant mice such that the difference between the groups became even more pronounced (Fig 8B, Open Field, p<0.001; Hole Board, p<0.001).
However, the response pattern for naked mole-rats differed from that of mice such that at the highest dose administered the withdrawal latency remained sensitized for the entire 120 minutes of post-SP testing.
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