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Experimental results indicate that the biosensor is able to detect recombinant H5 HA at 1.4 μM in 10% mouse serum, with high specificity for H5 as compared to H1 (H1N1 A/South Carolina/1/18).

We probed RNA from mouse serum with oligonucleotides complementary to 5′ or 3′ ends of specific tRNAs.

To establish a standard curve, mouse serum with known concentrations of HB22.7 were used for FACS analysis of the Raji cell line.

Therefore, the occurrence of autoantibodies against fractalkine was studied by testing the reactivity of mouse serum with blotted brain lysate containing the 31 kDa form of fractalkine.

Pooled polyclonal mouse serum with known anti-CII antibody concentration was included to each test plate and served as a positive control and standard.

We also probed RNA from mouse serum with a probe complementary to the 5′ end of tRNA-Asn-GTT to confirm the low abundance of circulating tRNA halves derived from tRNAs that were barely detected in the sequencing data.

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In an independent experiment, mice were immunized with 10 µl of anti-A172 mouse serum absorbed with rat anti total mouse immunoglobulin fraction (5 10 µl) and diluted to 100 µl with sterile saline.

Our results demonstrated that the sdAb-Zag fusion protein was highly expressed and specifically recognizes human, rat and mouse serum albumins with affinities in the nanomolar range.

However, in FD-deficient mouse serum treated with CVF in vitro no cleavage of FB occurs.

At higher concentrations of serum, the differential binding between NOD and Swiss mouse serum increased with a maximum differential of about 8-fold realized at ∼125 μg mL 1.

Mouse serum immunized with or without naked Ad was harvested at 14 days after second injection and incubated at 56 °C for 45 min to inactivate blood complement and stored at −20 °C.

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