Sentence examples for mouse plasma with from inspiring English sources
Here we used a mouse model of epithelial ovarian cancer in combination with the IPAS proteomic platform to allow us to reliably assess and quantify changes encompassing low abundance proteins in mouse plasma with tumor development [53].
Pathway analysis of the 48 proteins that were found to be upregulated in mouse plasma with tumor development, but not identified as secreted or surface membrane proteins in ovarian cancer cells yielded three significant networks with Ingenuity (Figure S4, Table S5).
A series of standards diluted from mouse plasma with known albumin concentration was run on the same gel.
Calibration standards, ranging from 5 nM to 100 μM final sunitinib concentration, were prepared by spiking 100 μl samples of control mouse plasma with sunitinib.
To evaluate successful engraftment, human albumin was quantified in mouse plasma with an in-house ELISA (Bethyl Laboratories Inc., Montgomery, TX).
The stability of labeling was tested by incubating 99mTc-labeled AuNP3 in the presence of 75% mouse plasma with subsequent analysis of samples using a Superose 6 10/300 GL column.
The calibration curve was obtained with pooled mouse plasma spiked with known amounts of the stock solution.
We compared the mouse plasma data with data from an extensive proteomic study of three human ovarian cancer cell lines (OVCAR3, CAOV3, and ES2) and of tumor cells from ascites fluid obtained from an ovarian cancer patient [15].
By comparing the upregulated mouse plasma proteins with the list of proteins enriched in the surface or secreted cellular compartments from human ovarian cancer cells (Table S2), 55% (58/106) of upregulated proteins in mouse plasma were found to be released from ovarian cancer cells through secretion or shedding or enriched in the ovarian cancer cell surface compartment.
In a search for potential ovarian cancer biomarkers, we performed a novel integrative analysis by comparing mouse plasma proteome data with human cell line and ascites derived tumor cell data.
Mouse plasma was mixed with 20 μL of internal standard (digoxin, 10 μg/ml).
