Sentence examples for rat serum with from inspiring English sources
Cell suspension was blocked 15 min on ice in PBS EDTA 2 mM 3% rat serum with anti-CD16/CD32 (SeroBlock, Abd Serotec).
For evaluation of keratan sulfate, we digested rat serum with Keratanase II and then measured the sum of monosulfate and disulfate disaccharides derived from keratan sulfate by HPLC.
TNF-α, interleukin-1alpha (IL-1α), interleukin-1beta (IL-1β), interleukin-6 (IL-6), interleukin-10 (IL-10), C-X-C motif ligand two (CXCL-2) and interferon gamma (IFN-γ) were measured in rat serum with a Luminex performance assay (RnD Systems, Minneapolis, MN, USA) according to the manufacturer's instructions.
The hydrodynamic size and zeta potential of the NPs were analyzed in PBS containing 5% rat serum with a Brookhaven 90Plus particle size analyzer (Brookhaven Instruments Corp., Holtsville, NY, USA) and a Zetasizer-Nano ZS instrument (Malvern Instruments Ltd., Malvern Hills, UK), respectively.
Antigen-specific rat T cells (TMBP and TOVA cells) were co-cultured for 48 h in 96-well plates (in Dulbecco's modified Eagle's medium 1% rat serum) with irradiated professional thymic antigen presenting cells as previously described (Flügel et al., 1999), in presence of specific or control antigen (10 µg/ml MBP or OVA) and BZ194 in DMSO, or DMSO alone.
Briefly, they were plated for 1 day in DMEM with 5% rat serum with 20 mM HEPES on millicell inserts coated with a mixture of 20% Engelbreth Holm Swarm tumor matrix (BD Biosciences) and 80% rat-tail collagen in DMEM and then cultured for 3 days with 1% CS-stripped FBS along with 10 ng/ml keratinocyte growth factor (R&D Systems Inc., Minneapolis, MN, USA).
The ability of meningococci lacking expression of fHbp and NspA to cause invasive disease in human fH transgenic rats and to survive in wild-type infant rat serum supplemented with human fH indicates an additional human fH-dependent mechanism of evasion of innate immunity.
Conceptuses were harvested at 7.5 dpc and cultured for 48 hours in pooled rat serum supplemented with 20 µm of the nitric oxide donor NOC-18 (2,2'- hydroxynitrosohydrazino bis-ethanamine) (Calbiochem), 10 pg/ml rVEGF (Chemicon), 2,2'- hydroxynitrosohydrazino bis-ethanamineCalbiochem
Electroporated embryos were cultivated in Rat serum supplemented with glucose as above, for the indicated time.
Cells were permeabilised, next blocked with rat serum and stained with anti-FOXP3 Alexa Fluor 488 (BioLegend) and relevant isotype rat controls.
PLN sections were fixed with 4% paraformaldehyde, rehydrated in PBS, and blocked with rat serum, prior to incubation with PE-conjugated anti-IgM (clone II/41; eBioscience, San Diego, CA, USA) and anti-LYVE-1 (Abcam17, AbCambridgeridge, MA, USA) together with secondary antibody FITC-anti-rabbit IgG (Invitrogen, Carlsbad, CA, USA) or PE-anti-rabbit IgG (Invitrogen).
