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To extend these findings on mouse genes, we searched the human genome database for orthologs.
To assign the probe sets to their corresponding human or mouse genes we used the mapping available in Bgee.
To examine further the relationship between mRNA levels and translational features observed for mouse genes, we similarly analyzed human gene expression data that was downloaded from the gene expression atlas SymAtlas v1.2.3.
We have not yet defined the transcription initiation site(s) of the SaCD79α gene but, as with the human and mouse genes, we hypothesise that SaCD79α may also have multiple initiation sites.
Similar(56)
In the mouse gene we have found only one binding site for IRF3.
For each mouse gene, we retrieved phenotypic information from the Mouse Genome Database (Bult et al. 2008) ("MRK_Ensembl_Pheno.rpt" file downloaded on 7 October 2010).
For each mouse gene, we retrieved the 2 kb upstream region based upon coordinates provided in UCSC ref gene files and sequences available from Bioconductor (package: BSgenome. Mmusculus.UCSC.mm10).
For each differentially expressed mouse gene we used the UCSC lift over tool together with chained BlastZ alignments to lift over the TSS +/-50 bp from mm9 to hg18.
To assess the level of expression of the endogenous gene and the transfected mouse gene, we used quantitative real-time PCR with primers to amplify nucleotides 439 541 of human WT1 (detects endogenous gene only) and nucleotides 1,071 1,207 of mouse WT1 (detects the transfected gene), respectively.
Finally, for each phenotype, we compared the associated human and mouse genes and we recorded all instances where the same orthologs were associated with the same phenotype in both species ("concordant orthologs").
To extend our search for volatility among the three families of mouse pheromone genes, we interrogated each gene region with the Mouse Paralogy Browser (She et al. 2008).
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