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To further illustrate the power of our RNA-seq data to uncover gaps in the mouse genome, we recapitulated genomic differences between founders for Mx1, an important immune gene.
To confirm the existence of the Xiap-ps1 gene in the C57BL/6 mouse genome we performed Southern blot analysis of genomic DNA from both wild-type (WT) and Xiap deleted mouse embryonic fibroblasts (MEFs), as well as from tissues from C57BL/6 WT and Xiap deleted mice.
To examine the potential genomic distribution of the miRNAs in the mouse genome, we utilized the web tool in miRBase (http://www.mirbase.org/) database to search the miRNAs by genomic location and the miRNA clusters based on base pair distance between neighboring miRNAs.
Inasmuch as alternative splicing potentially affects transcripts from more than two-thirds of the mouse genome, we suggest that these findings are representative of a genome-wide effect of ACTH that impacts on the mRNA and protein composition of the adrenal cortex.
When it's the mouse genome we're talking about, that probably puts Celera up against a company called Lexicon Genetics lexg.
In the mouse genome, we only found 53 miRNA genes completely located in SDs and two mmu-mir-3677, mmu-mir-367g) partially overlap with SDs.
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Since the Tmub1/HOPS amino acid sequences were highly conserved (89% identity) between the human and mouse genomes, we used mouse brain cDNA libraries for cloning the full-length gene.
To incorporate evolutionary conservation of genome regions of human and mouse genomes, we downloaded PhastCons [ 61, 62] conservation scores from the UCSC Genome browser.
When we plotted the density of the novel transcripts onto mouse genomes, we found that although novel transcripts were spread throughout the eight genomes, they did cluster around certain locations.
To investigate whether a particular transposable element (TE) mediated the duplication of SNORA70 in monkey and mouse genomes, we first searched for known TEs in the flanking sequences of SNORA70 using RepeatMasker [ 40].
Based on in silico simulation of enzyme digestion of human and mouse genomes, we have optimized the current single-enzyme RRBS by applying double enzyme digestion in the library construction to interrogate more representative regions.
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CEO of Professional Science Editing for Scientists @ prosciediting.com