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WT or NgR1 KO mouse forebrain were dissected and homogenized in RIPA buffer.
In the present study, mouse- and human embryonic stem (ES) cells and adult neural stem cells from the mouse forebrain were used.
To assess expression of control Retro-EF1α-EGFP vector (Figure 1A), isolated NSC from embryonic (E14) mouse forebrain were grown in the presence of rhEGF and bFGF, and dissociated cells were infected.
Mutant mice with targeted Atrx inactivation in the embryonic mouse forebrain were previously described (Atrx Foxg1Cre mice, defined as Atrx-null hereafter) (Bérubé et al., 2005).
We assessed whether the NPCs expanded as neurospheres from embryonic mouse forebrain were able to respond to cues from cerebral ischaemia.
When coronal slices of E12.5 Tg(Lrp12/Mig13a-Egfp Lrp12/Mig13a-Egfpwere treated with PP2 for 2 DIV, the temousel changes in Lrp12/Mig13a-positive cell shape and polarity seen in control slice cultures did not occur (Supplementary forebrain
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In the new Nature paper, Dr. Levitt and his co-workers described their studies of early neural development in mice, and how they learned that, starting at around day 10 of gestation — the equivalent of roughly the eighth week in humans — the mouse forebrain was flooded with serotonin, and the bath continued to what would be the gestational midpoint.
In mouse, forebrain is often chosen for behavioral and psychopharmacology research given its superficial similarity to human prefrontal cortex.
Briefly, mouse forebrain was dissected out, place in cold HBSS and chopped into ∼1-mm chunks with a sterile razor blade.
Deletion of Atrx in the mouse forebrain was achieved by mating Atrx loxP female mice with heterozygous Foxg1Cre knock-in male mice, as previously described (Bérubé et al., 2005).
Synaptic fractionations from mouse forebrains were prepared at 4°C essentially as described [32], [33].
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