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In order to be able to compare the anti-inflammatory activity of soy isoflavonoid genistein, which has already been confirmed by previous studies [9 11], to the one of genistein incorporated in HPBCD and RAMEB, inflammation was induced using the mouse ear model for inflammation and ear thickness was measured.
Moreover, inhibition of CAMP2 by neutralizing antibodies efficiently attenuated P. acnes-induced inflammation in the mouse ear model [ 45], suggesting that CAMP2, and probably the other ones as well, are virulence factors of P. acnes.
Functional inhibition of syntaxin-6 in vivo via adenoviral gene transfer of the inhibitory form of syntaxin-6 reduced VEGF-A-mediated angiogenesis in a mouse ear model [ 85].
Reynolds and colleagues [ 43] have demonstrated that in vivo expression of E-selectin during endothelial activation in an inflamed mouse ear model can be depicted with MR imaging and that the conjugate did not accumulate in non-inflamed endothelium.
It has been reported that DMSO induces viral reactivation both in the mouse ear model and in explanted ganglia [ 12- 16] and facilitates productive infections of Vp16 negative strains [ 17].
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It would be ideal to use a singe animal model but the inherent limitations for the chinchilla nasal colonization model and mouse ear infection model have prevented us from conducting these screens in the same animal model.
The anti-inflammatory activities of these compounds were tested using the xylene-induced mouse ear edema model.
Similarly, we analyzed the role of Jak3 in regulating LC migration using a mouse ear skin model.
Xylene-induced mouse ear edema model: Male ICR mice weighting 18 20 g were used.
Eupatorin has been shown to have anti-inflammatory effects in a mouse ear oedema model [ 7] as well as antiproliferative activity in human gastric adenocarcinoma (MK-1), human uterus carcinoma (HeLa), and murine melanoma (B16F10) cell lines [ 6].
Additionally, Piwnica et al 45 demonstrated that brimonidine tartrate displays anti-inflammatory properties by the inhibition of edema (when compared to vehicle) in in vivo mouse ear inflammation models.
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