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The anti-inflammatory activities of these compounds were tested using the xylene-induced mouse ear edema model.
Additionally, compound 7a blocked mice ear inflammation by 67% and suppressed the accumulation of inflammatory cells in an in vivo mouse ear edema evaluation.
In present study, xylene-induced mouse ear edema, carrageenan-induced rat paw edema, and carrageenan-induced mouse pleurisy were attenuated by esculin.
The 75% ethanol extract of the aerial part of E. splendens can significantly inhibit acute inflammation (mouse ear edema by croton oil-inducing) and subchronic inflammation (ear edema by phorbol ester inducing).
They also exhibited the significant anti-inflammatory activity in TPA-induced mouse ear edema assay and their anti-inflammatory effects were more than that of stellatin and indomethacin at 0.5 mg/ear.
In vivo, xylene-induced mouse ear edema, carrageenan-induced rat paw edema, LPS-induced mouse endotoxic shock, acetic acid-induced mice writhing and formalin-induced mouse pain models were used to evaluate the anti-inflammatory activity of IF.
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The results of mice ear edema depicted significant inhibition of ear edema (76.37 ± 12.52%; p < 0.05).
The alkaloid extract of CB (40 and 80 mg/kg) showed significant inhibition of xylene-induced mice ear edema as compared with the control, with inhibition of 32.1 and 45.1%% (P < 0.01) respectively (Fig. 1).
Anti-inflammatory activities of GWE were tested using rat carrageenan-induced hind paw edema and mouse xylene-induced ear edema experimental models.
The anti-inflammatory activity was investigated using acute topical edema of the mouse ear induced by xylene, acute paw edema induced by agar in rats, formaldehyde arthritis in rats, vascular permeability induced by acetic acid in mice and heat- and hypotonicity-induced haemolysis of ox red blood cells (RBCs).
Remarkably, 7-hydroxy-3,4-dihydrocadalin has also demonstrated anti-inflammatory properties on the croton oil-induced edema test in mouse ear [ 22] and carrageenan-induced paw edema model [ 18].
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