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Control electron microscopy experiments using a GABAB2 knock out mouse brain revealed no labeling.
For example, genome-wide analysis of Ago-binding sites in mouse brain revealed that 27% of the identified sites are "orphans," lacking a perfect seed match to the 20 most highly expressed miRNAs.
Moreover, transcriptome analysis in AICD transgenic mouse brain revealed no apparent difference between transgenic animals and littermate controls [ 20] and qPCR analysis of proposed target genes, including those studied here, failed to detect significant changes in mRNA expression.
Hierarchical clustering analysis of the splice variant expression across the mouse brain revealed two major groups with regional co-regulation: one contains variants with AS4 insertion, the other containing variants with AS3 insertion.
Detailed expression pattern analysis by immunohistochemistry in adult mouse brain revealed that MSK1 is mainly present in striatal and olfactory tubercle neurons as well as in hippocampus, exhibiting nuclear localization in all expressing cells, consistent with its function as histone H3 kinase [51,52].
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In situ hybridization of mouse brain reveals mRNA expression at P7 in the hippocampus, thalamus, cerebellum and cortex, and very low expression in adult brain (http://www.stjudebgem.org) [17].
Our discovery that the expression of several ancestral PAR1 genes is controlled by ATRX throughout the early developmental period of the mouse brain reveals an unexpected association between the levels of ATRX protein and the expression of these ancestral PAR1 genes.
Comparison of the transcriptome in narcolepsy and narcolepsy model mouse brains revealed a novel dysregulated gene which colocalized in hypocretin cells.
Electron microscopic analysis of 3-month-old Cln1/5 dko mouse brains revealed marked accumulation of GRODs within cortical and thalamic neurons (Fig. 2C, panels 1 and 3).
Postmortem immunohistochemical analysis of APPswe mouse brains revealed the presence of relatively few diffuse plaques in the cerebellum, which also differed morphologically from those in the cortex and hippocampus.
Detailed imaging of ECs in 2-year-old (2Y) mouse brains revealed MARCKS loses its apical clustering and becomes less polarized in the cytosol, whereas p-MARCKS is sparsely distributed (Fig. 1B; Movies S4 and S5).
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