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(Honorable Mention: By Mouse and Frog by Deborah Freedman Croww Made a Friend by Margaret Peot; Crunch by Carolina Rabei; Imaginary Fred by Eoin Colfer, ill. Oliver Jeffers; Juna's Jar by Jane Bahk, ill. Felicia Hoshino; Lenny & Lucy by Philip C. Stead, ill.

Our results from mouse and frog, taken together, suggest a conserved function for PCP in establishing L-R asymmetry in vertebrates.

The functional importance of VANG in regulating PCP in the Xenopus GRP also highlights the likelihood that the evolutionarily conserved mouse and frog nodal structures share this mechanistic feature required for embryonic lateralization [10].

The SUV39H2 gene is found in tetrapod (e.g. human, mouse and frog) but not in zebrafish (Figure 3A), suggesting that this gene is likely generated by a tetrapod lineage-specific duplication event.

The normalized gene expression data for mouse and frog were downloaded from the functional landscape of mouse gene expression website (http://hugheslab.med.utoronto.ca/Zhang) and the Conservation of Core Gene Expression in Vertebrate Tissues: Supplementary Data website (http://hugheslab.ccbr.utoronto.ca/supplementary-data/vertebrate_expression), respectively.

While Latimeria possess sister clades to all mouse and frog t2r genes, they have additionally retained a 'fish-like' taste receptor, unlike mouse and frog.

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In the neural tube of the mouse, chicken, and frog, YAP is expressed in the ventricular zone progenitor cells and co-localizes with the neural progenitor cell marker Sox2.

Conservation of gene expression was compared by checking the 3,892 1∶1∶1 orthologous genes in mouse, chicken and frog (Table S5).

Observations in cultured cells, as well as in mouse, zebrafish and frog embryos, have implicated IFT and basal body proteins in regulating the switch between canonical and non-canonical Wnt signaling [9] [11].

Besides testing conservation of gene expression of orthologous genes between species, we also tested whether homologous tissues (for example, brain tissues in mouse, chicken, and frog) are more similar to each other compared to non-homologous tissues.

Secondly, for all downloaded gene expression survey data, the tissue samples surveyed in mouse, chicken and frog [11], [16], [17] are only a part of the whole organs, representing the average of millions of cells of several different types.

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