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Exact(6)

Finally, the sections were counter-stained in Mayer's hematoxylin, dehydrated, cleared, and mounted for examination.

The slides were counterstained with hematoxylin, dehydrated, and mounted for examination.

Embryos released by dissection were incubated for ∼10 min, washed twice, and mounted for examination.

Finally, the slides were counterstained with haematoxylin and mounted for examination.

Samples were counterstained with 20% haematoxylin for 3 minutes and mounted for examination by light microscopy.

They were finally counterstained with DAPI and mounted for examination using a Nikon confocal microscope (Eclipse Ti, Nikon, Japan).

Similar(54)

The sections were then counterstained with Mayer's hematoxylin, dehydrated and mounted for microscopic examination.

After 30 min incubation at 37°C, cells were rinsed twice in PBS and incubated with DAB (Vectorshield) for around 10 min. Cells were washed in distilled water and mounted for microscopic examination.

Cells were washed twice in PBS, and fixed with absolute ethanol for 10 min at –20°C before addition of 100 µM of streptavidin HRP conjugate After 30 min incubation at 37°C, cells were rinsed twice in PBS and incubated with DAB for around 10 min. Cells were washed in distilled water and mounted for microscopic examination.

Sections were finally dehydrated, dried and mounted for microscopic examination.

The sections were then counterstained with Mayer's haematoxylin solution, dehydrated and mounted for microscopic examination.

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