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Subsequently, the sections were stained with 3, 3′-diaminobenzidine tetrahydrochloride (DAB) for 10 min, counterstained with Mayer's haematoxylin for 1 min, dehydrated and then mounted for light microscopy evaluation.
The slides were mounted for light microscopy with Clarion™ Mounting Medium (Sigma-Aldrich, St . Louis USA).
Cells were washed with PBS thrice, stained with 1% nuclear fast red to stain the cell nuclei, washed, air-dried, dehydrated, cleaned, and mounted for light microscope observation.
All slides were counterstained with Gill's hematoxylin for 1 min, dehydrated and mounted for light microscopic evaluation.
The slides were washed, dehydrated and mounted for light microscopy.
Samples were then mounted for light microscopy visualization.
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Sections were counterstained in weak Mayer's hematoxylin, dehydrated, cleared, and mounted for the light microscopy.
Sections were then counter-stained with Mayers hematoxylin (Histolab), then mounted with mounting medium for light microscopy (Pertex, Histolab).
Samples were counterstained with 20% haematoxylin for 3 minutes and mounted for examination by light microscopy.
The slides were then counterstained using Harris's hematoxylin, dehydrated, and mounted for evaluation via light microscopy.
Colour was developed with 3, 3-diaminobenzidine tetrahydrochloride and sections were counterstained with Gill's haematoxylin, dehydrated and mounted for evaluation on light microscopy.> In normal endometrium, the immunolabelling for ER-α, PR, Ki-67, CK7 and CK20 was evaluated independently in the surface epithelium (SE), superficial and deep glandular epithelium (SGE and DGE, respectively).
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