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The bottom row shows the motion corrected images using a non-rigid registration algorithm.

The remaining motion corrected images were registered in the order in which the retinal areas were imaged.

In certain areas of the motion corrected images, the connectivity of the smaller vessels may be difficult to observe.

Each of the 12 motion corrected images is shown in Fig. 12 along with the result of the automated mosaic algorithm.

After processing, the streak artifacts apparent in the raw image volumes were no longer present, and vessel visualization throughout the image was enhanced in the registered, motion corrected images.

The transform parameters of the GCL + IPL layer were then applied to the motion corrected images from the INL junctions to generate mosaics of the superficial and deep capillary plexuses in Fig. 13 and Fig. 14, respectively.

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Unlike conventional 4D PET imaging, motion freeze technology combines 100% of the PET counts into a 3D motion corrected image that has a comparable acquisition time and the equivalent image noise of a static acquisition.

The motion corrected image corresponding to the first region of the retina that was imaged served as the initial reference frame, I r x,y), for building up a mosaic.

Motion freeze technology combining the interpolated CT technology has high potential to overcome these challenges by using the entire acquired data to create a single 3D motion corrected image and to provide quantitative accuracy equivalent to 4D PET/CT with shorter acquisition times and less dose reduction [ 13, 14].

Prior to distortion correction, the data were motion corrected by image realignment with the reference scan.

Respiratory and cardiac motion corrected PET images using either M1 or M2 were compared to respiratory only corrected images and evaluated in terms of SNR and contrast improvement.

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