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The function with the greatest confidence was then chosen as the most likely annotation for that protein.
For each gene, the ten best significant matches in each database were conserved and reviewed for defining the most likely annotation.
The resulting matches Tj (where the index j denotes matches) yield a set of possible functions F = {fj }of S, and if one function f0 achieves plurality (recurs among Tj's more often than any other), then it is chosen as the single most likely annotation [76].
We then use the estimated factor loadings as observed variables in the CRF framework, so as to infer most likely annotation terms for previously unseen images.
The learned features are then used as observed variables in the CRF framework, so as to infer most likely annotation terms for previously unseen images.
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For these loci, we further investigated the most likely gene annotations by conducting a BLASTX search against proteins in the UniRef100 database (European Molecular Biology Laboratory European Bioinformatics Institute [EMBL-EBI], http://www.ebi.ac.uk/Tools/blastall/) (Table 1).
Low-scoring, taxonomically restricted orphans are most likely to be annotation artefacts: we tested this in the case of E. coli K12 by reference to online transcriptomic and proteomic expression data.
Moreover, even if one excludes singleton genes (those with no homologs in completed fungal genome databases), which are the ones most likely to be annotation artifacts, A. oryzae still contains 16 26% more genes than the other species: the numbers of non-singletons are 12,044, 10,425 and 9,574 for A. oryzae, A. nidulans and A. fumigatus respectively.
In making this decision, the curator takes into account information such as duplication events/orthology, sequence conservation, the presence of essential/active site residues, branch length, and genes having inconsistent experimental annotations (i.e. descendants with annotations, or missing annotations in well-characterized genes, that are most likely not compatible with the annotation).
Therefore, the cutoffs at 100% and 20% in Figure 5A and 5E represent the most likely scenarios for protein annotation.
However, this is most likely due to the annotation incompleteness and not a specific feature of the rat genome.
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