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Additional file 9: WaferGen SmartChip QRTPCR analysis of most divergent expression responses differentially expressed in RNA-Seq dataset.
Additional file 8: WaferGen SmartChip QRTPCR analysis of most divergent expression responses differentially expressed in Agilent microarray dataset.
The cytochrome oxidase loci are regulated in part by ArcAB in E. coli, which may explain why D. dadantii shows the most divergent expression patterns.
We compared 40 SAGE-derived transcripts with the most divergent expression levels between ROP- Os/+ versus C57- Os/+ kidneys, with the human microarray expression data, comprised of samples obtained from micro-dissected human kidney biopsies from patients with FSGS [ 40].
Agilent genes showing the most divergent expression compared to WaferGen were Disp2, Gabbr1, Heatr5a, Wdr11, Zfp524, and Zfp846 (Additional file 8), while Sult2a7, Tlr5, and Uox were only identified as DEGs by RNA-Seq (Additional file 9).
The comparison of the age-dependent expression between the lines together with the analysis of co-expressed genes showing a significant line × age interaction indicated that the processes related to reproduction and stress response showed the most divergent expression patterns between the lines.
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The T6SS shows one of the most dramatic divergent expression patterns observed in our experiments.
Candidate markers of healthy ageing or extended longevity include genes belonging to the categories showing most divergent age-related expression between the Drosophila lines.
The most divergent views were expressed about Puck and Beiste's performance of "Mean".
All markers revealed that the Santa Clara population is the most divergent (mean divergence p = 2.9%).
As shown in the heat map representation (Fig. 4), most of the GmARFs exhibited divergent expression profiles in the eight tissues examined.
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