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Exact(4)
The scale parameter μ determines the most common transcript number, and the shape parameter σ determines the width of the peak about the most common transcript number.
For each model cell we used values of μ such that the most common transcript number varied over a range from ~2 3 to ~100.
The COs were minimally 28,411 kb and maximally 28,964 kb apart and occurred in the third intron of the most common transcript of the rdgA gene, which is approximately 94 kb long.
From this we conclude that although we cannot determine the basic cell model with any accuracy, we can estimate the probable transcript distribution for cells of the type in this study as being that for which the most common transcript number ~8 and the number of genes with that transcript number ~500.
Similar(56)
A normalization step was carried out with the TRIMMER cDNA normalization Kit (Evrogen) in order to prevent over-representation of the most common transcripts.
A normalization step was carried out with TRIMMER Kit (Evrogen, Moscow, Russia) in order to prevent over-representation of the most common transcripts.
For the long read length assembly, read usage is overall lower and drops significantly as abundance increases: only 4 10% of reads are assembled from the most common transcripts.
In order to prevent over-representation of the most common transcripts, the resulting single-stranded cDNAs were normalized using the Kamchatka crab duplex-specific nuclease method (Trimmer cDNA normalization kit, Evrogen) [ 69].
Because of the transcription gradient in the Mononegavirales, the most common primary transcript is NP [ 13].
Functional profiles of the most common nuclear transcripts in oocyte, 1-2 cell, blastula and gastrula (obtained from the use of gene ontology (GO -terms) revealed that both within cellular component, molecular function and bioloGO -termscess many of the trevealedthatid not change much in their abothance over time (data not showithin
We tested if the frequency of the two most common ESR1 transcripts (wild-type and Δ7 ESR1) differed in controls and patients with major mental illness, and we found that the frequency of wild-type ESR1 mRNA expression was lower in patients with schizophrenia (80%) when compared with normal controls (97%) (χ = 3.86, df = 1, P < 0.05) (Fig. 5, Table 7).
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