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The system could potentially be further improved by enhancing the physiological relevance of the tissue compartments themselves (e.g. the use of more relevant cell systems, implementation of more appropriate tissue architecture).
Cell engraftment inside the scaffolds was assessed by SEM microscopy and histology, evidencing more relevant cell migration and production of extracellular matrix in C-RGD versus G-FOAM.
First, we tested whether EBV LMP1 could also induce EMT in this more relevant cell system.
While the majority of reported PEMF-induced effects were studied in osteoblasts, findings in literature indicate that MSCs could be a more relevant cell source in vivo.
For clarity and consistency the authors should also show growth curves for virus in knockdown and control cells and repeat these experiments in one other more relevant cell line (HeLA cells are very particular).
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Starting with cells from patients with spinal muscular atrophy, predicts Ipierian Chief Executive John Walker, will enable his scientists to test potential drugs against far more relevant cells than have been used before.
However, these studies were performed with common laboratory cell lines, and information on the impact of ceramide as an inducer of programmed necrosis in clinically more relevant tumor cell systems is currently unavailable.
These results demonstrate that parameters such as ratio between size and inter-particle distance can be more relevant for cell response than wettability on nanostructured surfaces.
Finally, our study has confirmed and extended the role of ceramide as one of the key mediators of programmed necrosis downstream of RIPK1 and RIPK3 to the clinically more relevant tumor cell systems investigated here, with the A-SMase inhibitor Arc39 additionally validating A-SMase (rather than neutral sphingomyelinase or ceramide synthase) as the main enzyme responsible for ceramide generation.
Creation of more physiologically relevant cell models in tissue culture is a requisite for advancing medical research.
This fabrication method provides new capabilities to manufacture structurally and mechanically relevant cytocompatible scaffolds that will enable more physiologically relevant cell culture studies.
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Justyna Jupowicz-Kozak
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