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Instead, HEK293T is a more appropriate cell line for such screens since it can be easily and effectively transfected and transduced and shows negligible proliferation defects upon acute, partial silencing of FH.
The advent of improved methods of human cell immortalisation, combined with the use of pre-separated characterised cells, does enable more appropriate cell line models to be developed [ 40].
Although reviewer 2's suggested rescue experiment and the need to repeat the Ago experiments in a more appropriate cell type could be excluded if the authors are able to address the above 3 key points, the other specific points seem within the authors' grasp of what could be carried out in a reasonable length of time.
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But he added that if the project reached the clinical stage, perhaps in two years, it would be necessary to derive new and more appropriate cells.
This will allow researchers to both produce more appropriate cells for transplantation, and to explore the mechanisms underlying 'disease in a dish' models of disease through tagging specific proteins with markers and producing isogenic control lines where possible.
Ultimately, this knowledge will allow refinement of the current differentiation protocols and derivation of well-defined, and thus safer and more appropriate, cells for replacement therapies [ 3, 108- 110].
Taking these points together, a more appropriate NP cell culture system and the role of macrophages in immune privilege of NP cells should be further explored.
Electrospun nanofibers provide high porosity with large surface area-to-volume ratio and are more appropriate for cell accommodation, nutrition infiltration, gas exchange and waste excretion.
The Hoechst dye thus appears to be a more appropriate stem cell marker.
There are 2D sequences of nuclearly stained cells, commonly used in cell population studies (e.g. N2DL-HeLa), and 3D sequences of cytoplasmically stained cells, more appropriate for single-cell studies that demand a realistic rendering of the cellular environment (e.g. C3DL-MDA231).
We interpreted these data that AVP neurons are lost through 'autophagy-associated cell death', a term that is more appropriate than 'autophagic cell death' because of the original role of autophagy in the AVP neurons.
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