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Introduction of quantitative proteomics approaches, such as isotope coded affinity tags (ICAT), allow obtaining more precise protein abundance estimates for a broader range of protein concentrations.
One of the aims of this study was to identify biomarkers that discriminate diseased from healthy fetuses using the proteomic dataset as a guide for candidate proteins that require follow up with more precise protein chemistry methods (Western blot, immunoflourescence microscopy, and explant assays).
UniProtKB/Swiss-Prot annotates exclusively proteins and has more precise protein annotations than dictyBase does.
Using immunofluorescence for the protein of interest, in combination with other fluorescent markers for particular cell landmarks (e.g., DNA), allows for more precise protein localization.
To further confirm the repressive effect of these miRNAs on PTC -APC mRNA in its natural sequence context, we constructed minigene vectors that express both an HA-tagged PTC- APC bearing either a wild-type miRE or a mutant miRE with mismatches in the seed region and an HA-tagged EGFP that served as an internal control for more precise protein quantification.
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More detailed and precise reconstruction of DPR network evolution requires more accurate protein phylogenies, complete information of protein functions and domain architectures in multiple species, and a more comprehensive and complex model to characterize the aforementioned mechanisms of network changes.
A more precise measure of protein expression is provided by experimental quantification of the abundance of individual proteins, such as has been performed in S. cerevisiae for a largely complete set of encoded proteins [ 24].
These results suggest that tagged versions of Rn are more precise in reporting protein dynamics and the GAL4-driven GFP perdurance masks the temporal expression pattern of endogenous Rn protein.
For more precise detection of protein complexes, we used the characteristics of bottlenecks in the network.
In FICP, cells are diredly fixed after incubation with cydopentenone allowing a more precise detection of protein levels.
Such an approach allows for the identification of negative tumour nuclei from stromal elements, thus allowing for a more precise determination of protein expression.
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