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Exact(19)
Six to ten clones were sequenced per individual when sequences contained two or more polymorphic sites.
Obviously, increasing θ will produce more polymorphic sites in DNA samples and increase the power to detect ancient recombination events.
Each genome requires the testing of one or more polymorphic sites to pull it out from the other genomes.
Consecutive stretches of 10 or more polymorphic sites (equivalent to at least 350 bp), all showing reduced signals, were classified as deletions.
However, in this study, only three (EST13, EST24, and EST 28) out of 8 genes showed 2-fold more polymorphic sites in noncoding regions than in coding regions.
Most genes (8,019) contained at least one polymorphic site, and about 35% of them had four or more polymorphic sites (Additional file 1: Table S3).
Similar(41)
Because of a program limitation, that it cannot handle more than 1000 polymorphic sites at once, we performed the analysis separately on two exact halves of the concatenated product (comprising of the multilocus sequences).
To improve reliability of the estimation, only transcripts with more than 9 polymorphic sites were analyzed.
Fragments shared by a pair of DNA sequences in a multiple alignment presenting more consecutive identical polymorphic sites in common than expected by chance are identified.
Of these, one parasitism related transcript (contig43771 encoding a protein histidine kinase) exhibited more than 9 polymorphic sites and was under positive selection.
These questions can only be answered when more information on polymorphic sites and cancer-related mutations in the IL-6 gene will be available.
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