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SRAP primers produced more polymorphic bands per combination (18 vs. 11.5), but similar rates of polymorphism (∼95%).
The percentage of full methylation at CCGG recognition sites was slightly higher in somaclones due to more polymorphic bands generated after cleavage by EcoRI/ HpaII.
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The additional, smaller and more polymorphic interstitial bands detected are most likely to be composed of other tandem repeat(s), some of which might be cytotype specific.
Each of these primers was able to demonstrate one or more differentiating polymorphic bands in the resistant and susceptible strains in B. alexandrina.
The advantage of RAPD relies on its simplicity, rapidity, a small quantity of DNA, and its ability to generate more number of polymorphic bands, numerous polymorphisms [ 24], and the observation of the specific band pattern from each primer [ 25].
E. grandis showed more fragments and polymorphic bands, indicating that LTR-RTEs families had distinct activity after speciation events in this specie.
Relative to other markers employed, SRAP primers yielded more than twice the number of polymorphic bands per primer and also demonstrated the highest average polymorphism information content (PIC).
Results showed that RAPD analysis using 21 primers yielded 279 bands and 86 polymorphic bands (31% polymorphism).
The AFLP-based analysis appears more robust because it was based on 363 polymorphic bands while only 88 signals were evaluated in the metabolic profiles.
More than 70%% of the 913 primer pairs produced clear polymorphic bands between the P- and G-type plants.
Only 2 'Syrah 174' and 5 'Chardonnay 96' somaclones showed ten or more variant bands, whereas the majority (45/78) exhibited less than 4 polymorphic bands.
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