Sentence examples for monitored the subcellular from inspiring English sources

To follow up on the dual mode of SHARP protein function in more detail, we used mammalian two-hybrid assays (Figure S7) and monitored the subcellular localization of NPAS2 upon co-transfection with either BMAL1, SHARP-1 or SHARP-2 encoding expression plasmids (Figure S8).

For this, we monitored the subcellular localization of Hsp104, an aggregate-remodeling chaperone, in wild-type and ncs2Δelp6Δ cells upon diamide exposure.

To this end, we stained asynchronously growing HeLa cells with specific antibodies, and monitored the subcellular localization of the proteins in interphase and M phase.

To further expand on the role of STK38 in autophagosome formation, we monitored the subcellular localization of ATG14L, WIPI-1, and ATG12.

We monitored the subcellular distribution of the EYFP-Bad and EYFP-BadS136A fusion constructs by epifluorescent microscopy with and without co-transfected Akt-1 and could not detect altereded subcellular localizations (data not shown).

Monitoring the subcellular localization of the appropriate fluorescent fusion proteins validated this conclusion.

First, we used immunoblotting to monitor the subcellular distribution of Rab26 during the purification of synaptic vesicles from the rat brain.

We investigated if BAX aggregation was altered at non-lethal levels of fusion protein by monitoring the subcellular localization of GFP-BAX in HCT116 BAX-/ cells, before and after, indomethacin treatment.

Importantly, MarsCy1 fusion did not perturb normal Lgr5 trafficking (Additional file 3: Figure S3 and Additional file 4: Figure S4) and could be used in combination with SCi1 to monitor the subcellular distribution of Lgr5 and its internalization by confocal microscopy (Fig.  1).

To test the causes behind the low transcriptional activity of β-catenin in mESCs, we monitored the abundance and subcellular location of β-catenin and of some phospho-specific forms during self-renewal and differentiation.

Because heat stress has been previously shown to cause mislocalization of FUS, we applied brief periods of thermal stress (35°C for 30 min) and then monitored both the subcellular distribution of FUS and the severity of motor dysfunction.