Sentence examples for observe the subcellular from inspiring English sources
Meanwhile, in order to observe the subcellular localization of Cht1SP-thanatin(S -GFP and thanatin(S -GFP, the sequences of Cht1SP-thandthanatinnd thanatin(S -GFPe reS -GFPvely linked thepGWB5 (with GFP tag).
To observe the subcellular localization of OsSND2, a green fluorescent protein (GFP) fused to the C-terminus of OsSND2 and inserted into the pCAMBIA1300 between the KpnΙ and BamHΙ sites to create the 35S::OsSND2-GFP vector, which was transformed into rice protoplasts by polyethylene glycol (PEG) mediated transformation method.
Confocal microscopy was applied to observe the subcellular localisation of NDRG2 and GLUT1 in SK-BR-3 cells.
To observe the subcellular location of IFIT4, THP-1 cells were transfected with pEGFP-IFIT4 or pEGFP-C1 empty vector.
Given the sequence divergence between human ASAP and X-ASAP, we overexpressed the full-length EYFP-X-ASAP in Xenopus XL2 cells to observe the subcellular localization.
To observe the subcellular localization of W10G11.20 in adult gonads and early embryos, we generated transgenic lines that express GFP::W10G11.20::FLAG under the control of the pie-1 promoter that drives transcription in the germ-line.
To improve this delivery system and to follow the destiny of exogenous protein, we observed the subcellular distribution of transduced gp91phox 90-N protein in HeLa cells after 8 and 24 hours by immunocytochemistry using either an anti-his antibody (Figure 6B) or an anti-gp91phox antibody (Figure 6C).
To determine if TcNPR1 can also translocate into the nucleus in response to SA in a manner similar to Arabidopsis NPR1, we created transgenic Arabidopsis plants containing a TcNPR1-EGFP translational fusion and observed the subcellular localization of the fusion protein using confocal microscopy.
In addition, we observed the subcellular location of DnSIZ1 and the fluorescent signal in roots derived from the transgenic plants showed that DnSIZ1 protein accumulated in the nucleus (Fig. 5), which is further evidence for DnSIZ1 function as a SUMO E3 ligase in Dendrobium.
To further confirm our results, we observed the detailed subcellular structure in dsRNA-treated PSG by electron microscopy.
In order to observe the impact of number of subcellular locations we constructed two more datasets of nine and five subcellular locations.
