The leaf was used to monitor expression of the three cloned genes.
These constructs were used in different vector backgrounds in which the presence or absence of GFP allowed us to monitor expression of the shRNAs (see MATERIALS AND METHODS).
To ensure that detection of impairment of the UPS in R6/2 brains was not obscured by dysregulated expression of the GFPu transgene, quantitative real-time RT-PCR analysis was carried out to monitor expression of GFPu mRNA.
A phylogenetic study and qRT-PCR assays were developed to globally monitor expression of type 1 and 2 cystatin genes in normal mouse tissues and to gain insight into potential compensation mechanisms involved in Csta/Stfa2l1 mutant tissues.
Planktonic cultures of E. coli BW25113/pgadX-gfp and BW25113/puspE-gfp were used to monitor expression of gene gadX and gene uspE in response to the exposure of hydrogen peroxide.
The second construct is a reporter mRNA which consists of eight binding sites (19-base stem-loop) for the MS2 coat protein, a ß-galactosidase coding sequence to monitor expression of the construct and the SV40 3'UTR (Untranslated Region) to mimic the behaviour of non-targeted mRNAs.
