It was therefore the aim of the study to screen expression of metabolic proteins mainly represented by metabolic enzymes in the hippocampus over the estrous cycle and in males.
To this end, we first screened expression of ERBB receptors in mouse MSCs and determined that NRG1 and ERBB2, but not ERBB4, were expressed in MSCs.
We have set up an experimental strategy that allows us to screen expression of genes in up to 112 different human tissues of multiple developmental stages and cell lines.
Next, we screened expression of SRC1, TIF2, PIAS1, PIASx, ARIP4, BRCA1, β-catenin, AIB3, AIB1, CBP, STAT1, NCoR1, AES, cyclin D1, p300, ARA24, LSD1 and BAG1L coregulators in triplicates (three independent Q-RT-PCRs' runs).
To test if miRNA pathways crosstalk with the pharmacologic action of bufalin in cancers, we screened expression of some cancer-related miRNAs in PC-3 cells after bufalin treatment, and observed miR-181a expression to significantly increase in a dose-dependent manner.
In our previous study, we screened expression of different miRNAs in archived formalin-fixed and paraffin-embedded breast tissue specimens by in situ hybridization [ 10, 28], we found significant downregulated miR-133a expression in breast cancer compared to benign breast disease and miR-133a downregulation was associated with disease progression.
To be detected in our screen, expression of the cDNA might affect the microtubule inhibitor-mediated cell death/senescence pathway either in a dominant or dominant-negative manner.
The intein-GFP system may provide a simple route for monitoring real time soluble protein expression, predicting final product yields, and screening the expression of a large number of recombinant proteins for rapid purification in high throughput applications.
The stable clone was verified by screening the expression of the targets using Western blots.
In libraries made in the pG8SAET-vector, an increase in the number of correct clones can also be followed by screening for expression of the E-tag.
