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Next, heparin molecules were conjugated to the reactive surface of the scaffolds.

The homing molecules were conjugated at the end of the lipid, and they specifically bind to their receptors on the breast cancer cell surface.

Heparin molecules were conjugated on the fiber surface up to 112.4 ± 3.2 ng/cm2, and recombinant basic fibroblast growth factor (bFGF) was loaded onto heparinized fibers up to 96.3 ± 4.7 ng/mg scaffold.

Covalently linking enzyme molecules on the surface of MOF particles (Shi et al. 2016; Cao et al. 2016) was reported as another effective way for enzyme immobilization on MOFs, where the enzyme molecules were conjugated to the carboxylate groups on the MOFs.

In order to improve the efficiencies of polyamine-β-cyclodextrin-based cationic polyrotaxanes (PR-EDA and PR-DETA) as DNA condensation materials, we have designed and prepared two novel scutellarin-grafted cationic polyrotaxanes (PR-EDA-SCU and PR-DETA-SCU), in which scutellarins (SCU), the planar molecules, were conjugated on the cyclodextrin molecules of PR-EDA and PR-DETA.

It should be noted that PEG molecules were conjugated to GFLG in order to easily detect the proteolysis of GFLG based on the frequency shift due to mass of PEG (for details, see below).

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When such targeting molecules are conjugated to drug delivery nanoparticle, more of the anticancer drug intends to enter the tumor cell, and increase the efficacy of treatment along with reduction of toxic effects on surrounding healthy tissue.

Ubiquitin can form multi-ubiquitin chains, in which the first ubiquitin is attached via an isopeptide linkage to a lysine of the target protein and the subsequent ubiquitin molecules are conjugated to lysine 48 of the preceding ubiquitin molecule.

(1317) The products of the Phase-I reactions or the unchanged molecules are conjugated with hydrophilic endogenous molecules (e.g., amino acids, glutathione, glucuronide, sulfates) in the Phase-II reactions.

The MALDI-TOF profiles in Figure 5a show the mass shifts compared to BSA alone, indicating that 1.5−2 glycans/protein molecule were conjugated.

Then, GstB catalyzes the rate-limiting step whereby a second glutathione molecule is conjugated to the intermediate complex.

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