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SERB assisted with genotyping and molecular verification of Cre expression.
A total of 15 maize lines were used for molecular verification of this HelitronFinder prediction (Fig. 6).
The importance of the molecular verification of such hypothesis cannot be overemphasized, since QTL pyramiding is at the core of the current genomics-assisted breeding paradigm [ 5].
The 18S rDNA amplification was performed with general nematode primers commonly used in molecular verification of D. medinensis to confirm that samples were not morphologically atypical guinea worms.
The present case extends the significance of the genus Fusarium as agents of keratitis and underscores the utility of molecular verification of these emerging fungi in the human host.
After the experiment, to obtain molecular verification of nematode infection, we performed DNA extraction and subjected the DNA to PCR, according a protocol described by Rahmanet al. [ 55] (Additional file 1: Figure S9).
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He advises his students and postdocs to get experience in as many areas as possible, "from engineering to statistical analysis to molecular verification," and to do this as part of their project.
Strains are no longer accessible for molecular verification.
We randomly selected five predicted Helitrons from the program's high quality output for molecular verification.
The present case extends the significance of genus of Fusarium as agents of keratitis and underscores the utility of molecular methods in verification of these emerging fungi in the human host [ 29].
The implementation of this inverse problem under a molecular dynamics framework provides verification of this methodology.
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