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Molecular validation of APIP12 knockout mutant.
Molecular validation of our bulk density based analysis to determine Vc, is achieved by mapping to the eight-chain network model of Arruda and Boyce.
Finally, molecular validation of CNVs, using a variety of independent methods, indicated that approximately one third of the CNVs detected by the 2.1M array were false positives.
We next assessed the false positive rates in aCGH by independent molecular validation of a subset of CNV regions (44 deletions and 17 gains).
Cellular and molecular validation of this model was conducted in three phases.
F1 and R5 indicate the sites were the primers bind, which were used to amplify exons 1 5 for molecular validation of morpholino efficiency.
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These deletions were grouped by strain, and then categorized as 'simple'complexlex' and 'false positive', depending on the molecular validation result of the corresponding CNV region (Figure 5A and B, and Figure S2 A to E).
In general, molecular validation is necessary to confirm the presence of CNVs.
In addition, as racial differences may also exist in the expression of molecular markers, validation of these models should be performed in the following research, including different races.
The application of the various algorithms to datasets that do not include molecular validations will generate many false positives.
As the IE/FC method allows CTC isolation for molecular analysis, continued validation of this technique is of interest for future research.
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