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With the development of molecular techniques, however, it is now possible to employ useful biomarkers to identify subjects at high-risk who should receive this pathological screening test.
Secondary outcomes will incorporate aetiology information from standard culture and molecular techniques; however, these additional data will not serve as the gold standard.
These remarkable successes of molecular techniques, however, should not obscure the fact that a bacterial origin was previously established for both diseases on the basis of histologic studies and clinical responses to antimicrobial treatment (2, 48 ).
Identification of Pfiesteria-like organisms is a particularly difficult element of the monitoring programs, as these small species cannot be definitively identified using light microscopy; newly applied molecular techniques, however, are starting to provide alternatives to traditional methods.
Distinction between viral and bacterial pneumonia by microbiological and/or molecular techniques, however, is highly relevant in terms of initiating antimicrobial therapy, as 32% of patients with viral pneumonia develop a concomitant bacterial pneumonia [ 23].
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However, molecular techniques cannot distinguish between live or dead organisms, generating false-positive results (low specificity) and therefore being of little clinical utility [26].
(both are Anisakis type I); however, molecular techniques can easily distinguish the 2 types of larvae (3, 4 ).
However, molecular techniques are not precise enough to indicate which species, dogs or the 3 wildlife species, maintains the virus cycles that are found in wildlife.
However, molecular techniques have recently been developed as effective tools not only for the diagnosis of individual cases but also for studies of taxonomy and evolution of anisakid nematodes (3, 4 ).
However, molecular techniques used in this study can be adequate for an easier and faster epidemiological evaluation of S. aureus carriage in the general pediatric population in order to have useful information concerning the circulation of MRSA in the community and the risk of the occurrence of community acquired (CA)- S. aureus illnesses that are potentially difficult to treat.
In mollusks, sensory organs have been described by histological and immunochemical techniques, however, the molecular underpinnings of their development and evolution remain largely obscure (but see [ 9]).
Related(20)
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