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Viruses were not targeted by molecular methods; however, culture for cytomegalovirus (CMV) was performed on 79 samples.
Most of the recently described molecular methods, however, rely on culture amplification as a precursor to diagnosis (5– 8).
Sequence-based molecular methods, however, offer alternative approaches for microbial identification directly from host specimens found in the setting of unexplained acute illnesses, chronic inflammatory disease, and from anatomic sites that contain commensal microflora.
As a simple point mutation (or point polymorphism), the 677C→T variant is easy to screen for using molecular methods; however, homozygosity for this mutation accounts for only about a third of cases of hyperhomocysteinemia. Therefore, many authorities feel plasma homocysteine measurement is more informative than molecular testing.
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Following the development of molecular biological methods, however, a wide range of mucin structures became available and gave rise to a plethora of biochemical definitions of mucins.
The method seems to have many advantages over other molecular detection methods; however, it is too early to say whether the PLP assays would be used routinely to detect SRE bacteria in the future.
An orange stem pitting citrus tristeza virus (CTV) isolate CTV-B165 was found to be symptomatically similar to other known CTV-VT isolates however molecular methods failed to classify it as an identifiable CTV genotype.
However, molecular methods require expensive equipment and trained laboratory staff.
However, molecular methods have been shown to be more reliable for diagnosis (6, 7, 11 – 11 ).
However, molecular methods detect viable and non-viable bacteria, and do not indicate current infection/colonisation.
Testing of environmental samples for Coccidioides, Histoplasma, or Blastomyces has traditionally relied on culture methods and animal inoculation, which are associated with low sensitivity and which are labor and resource intensive; however, molecular methods such as PCR are promising (33 ).
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