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Exact(8)
In addition, the data clearly indicate that binding only occur with the expected molecular mass corresponding to the recombinant proteins (Fig. 3A).
After one hour of incubation, the band corresponding to the E6 associating protein (100 kDa) disappeared and bands of higher molecular mass corresponding to polyubiquitinated E6 associating protein could be detected for both (4R -FPro-ub and wt-ub (Figure 5).
The N-glycan at N266 had a molecular mass corresponding to a non-fucosylated pauci- or oligo-mannose structure [Man2-6GalNAc2].
IgG-Sepharose affinity column chromatographic separation of Z domain containing chloroplast derived IGF-1 protein confirmed a protein with a molecular mass corresponding to human IGF-1.
A band with a lower molecular mass corresponding to RyR3 (545 kDa) was not detected by mAb 34C from cultures at any time point examined.
We therefore established a refolding protocol that yielded pure protein eluting as a single peak in SEC with an apparent molecular mass corresponding to a monomer, indicating that the protein was folded in a globular shape.
Similar(52)
The quantification of the labeling was determined by calculating the relative peak height of the molecular masses corresponding to the chelated and unchelated peptide.
When identically prepared membranes were blotted with the two lectins, a cluster of spots with apparent molecular masses corresponding to the di- and the monoglycosylated forms of PK treated PrPSc was obtained with both lectins.
Upon blotting with 6H4 a cluster of immunoreactive spots covering a range of apparent molecular masses corresponding to the PK treated di-, mono- and unglycosylated forms of PrPSc was recognized.
Instead, the encoded proteins show calculated molecular masses corresponding to one BBP domain per polypeptide.
SDS-PAGE analysis indicated protein bands with molecular masses corresponding well to the theoretical values.
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