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Rhenium tricarbonyl conjugates of C2AH were analysed post tryptic digest by liquid chromatography-electrospray mass spectrometry (LC-MS), giving rise to a peak with the molecular weight corresponding to M+ = [Re(CO 3 + CK + LAAALEHHHHHH]+.
Comparison analysis between CR2 and the fusion protein on SDS-PAGE suggested that the fusion enzyme CR2-L-GDH was successfully constructed and expressed with the expected molecular weight, corresponding to the sum of both the theoretical molecular weights of CR2 (39.8 kDa) and GDH (28.5 kDa) (Fig. 3).
Two major protein products were found in cell lysates, with an apparent molecular weight corresponding to E and prM proteins.
On Western blot, this antibody recognizes a single band of approximately 60 kDa molecular weight corresponding to the TH protein (manufacturer's technical information).
Interestingly, a metalloendopeptidase with a molecular weight corresponding to that of meprin was previously found in rat glomeruli, but was not identified [50].
In cells grown at neutral pH, SsrA was detected as one band with molecular weight corresponding to the size of the mature form (386 nt).
Similar(30)
It is suggested, based on the limited data available, that a blend comprising two monodisperse LPE/BPE components, with molecular weights corresponding to the weight-average molecular weights of the polydisperse linear and branched polyethylenes, would not undergo liquid liquid phase separation.
The molecular weights corresponding to the peaks of MtbMfd and its derivatives were calculated from the standards graph using graph pad prism software.
Coomassie blue stained gels of the coupling reaction demonstrated the appearance of bands with molecular weights corresponding to those predicted for GIP peptides covalently linked to QβVLPs Coupling bands corresponding to one, two, three or four peptides coupled per subunit could be identified.
The major protein products obtained in this assay have molecular weights corresponding to the expected size for Env protein of D. melanogaster, about 54 kDa.
Different protein bands were observed for RGDPEG-avidin with molecular weights corresponding to the unmodified subunit and to subunits modified with one or multiple RGDPEG moieties.
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