Interestingly, in addition to these molecular clones corresponding to two duplicated genes, different forms of ARs, termed AR-A and AR-B, have also been biochemically characterized based on their binding affinities for different androgen ligands in the Atlantic croaker, Micropogonias undulatus [ 34] and the kelp bass, Paralabrax clathratus [ 35].
Restriction enzyme sites Xma I and Nhe I were introduced by silent mutations into the molecular clone at positions corresponding to HIV-1 RT codons 15 and 267, respectively [39].
This involved the isolation of ethylene response mutants, such as ethylene-insensitive or constitutive ethylene-response mutants, followed by the molecular cloning of the corresponding genes and the determination of their order of action.
In previous studies, we found that replacing domain III in an infectious molecular clone of GBV-B with the corresponding HCV domain (GB/IIIHC) resulted in an infectious genome with rescue of a chimeric virus following intrahepatic inoculation of RNAs into a susceptible tamarin, yet with an unusual delay in the onset of viremia.
The pol gene fragment (nt 4079-4342) corresponding to the 5103 fragment from the infectious proviral molecular clone pNL4-3 wamplifiedied by PCR.
Table 2 shows that the parental molecular clone, SHIV-1157ipEL, and the passaged biological isolate, SHIV-1157ipEL-p, are both tier 1 viruses, while the corresponding "late" virus, SHIV-1157ipd3N4, is categorized as tier 2 [1].
Each molecular clone was transfected into COS-7 cells.
The resultant replication-competent molecular clone was designated as p05MYKL045.1.
The resulting fragment was cloned at EcoRI and StuI sites in the 89.6 molecular clone.
† Represents clone corresponding to the largest EST.
