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The data suggest the existence of a large molecular machine, the "unwindosome," which separates DNA strands at the replication fork and contains Cdc45, GINS, and the MCM2-7 holocomplex.
ATP, the fuel of life, is produced in mitochondria of living cells by a molecular machine, the ATP synthase.
This process is mediated by an elaborate molecular machine, the spliceosome that consists of 5 snRNPs (small nuclear ribonucleoprotein particles) along with numerous less stably associated proteins, and is conserved throughout the eukaryotic world [ 1- 3].
This self-sustained time-keeping system is generated and maintained by an endogenous molecular machine, the circadian clock, which is a transcriptional mechanism composed of the transcription factors CLOCK and BMAL and their co-repressors, PER and CRY.
The introns are excised at the donor and acceptor splice sites such that the flanking coding regions, exons, are spliced by an extremely complex ribonucleoprotein molecular machine, the spliceosome [ 2, 3].
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For molecular machines, the main issue is the bond between molecules that is very weak.
Specifically, due to the size of these molecular machines, the resolution of the structures was too low to distinguish H atoms or even parts of residues.
Therefore, the origins of the single molecular machine of the nucleolus the eukaryotic SSU processome, are revealed more clearly in the present work.
By electron cryo-tomography of whole Thermus thermophilus cells, we determined the in situ structure of a T4P molecular machine in the open and the closed state.
Gene expression involves transcription, in which mRNA is copied from a DNA template, followed by translation, in which a molecular machine called the ribosome interprets the sequence of an mRNA to make the protein it encodes.
The nickel doesn't react with the oils, instead it acts as a molecular machine, enabling the oils to react with the hydrogen.
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