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Clones representing both variants (NbSerk3A, NbSerk3B) were identified based on high overall similarity to SERK3. A. tumefaciens GV3101 was used in molecular cloning experiments and was cultured at 28°C in lysogeny broth (LB) media using appropriate antibiotics.
UM performed the molecular cloning experiments.
Primer and synthetic gene sequences used in molecular cloning experiments are available on request.
Through the molecular cloning experiments in this work, the following suggestions may be made regarding PMM genes and chromosome loci in Triticeae species.
All of the common barriers to molecular cloning experiments of cost, faculty expertise, equipment, and limited class time are minimized by the pClone system.
BKL designed and conducted the experiments and the data analysis; AND performed molecular cloning experiments; SCC, and MYC performed other biochemical experiments.
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AR performed all molecular cloning, cell experiments, mechanical testing, and histology.
SPM carried out the molecular cloning and immunolocalization experiments.
ORF: Open Reading Frame; OST: ORF Sequence Tag; JGI: Joint Genome Institute LG designed the cloning experiments, carried out molecular cloning, 454 sequencing, sequence analysis and drafted the manuscript.
His team performed embryology experiments, molecular cloning projects, and candidate gene studies, coming at the problem from several different angles at once.
Ease in SBP marker development and application to any genomic regions, and genome-wide abundance of SNPs make this technology suitable for mapping experiments, especially to develop high density molecular maps for positional gene cloning experiments, if the assembled genome sequence and physical maps of the studied species are available.
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molecular cloning projects
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molecular biology experiments
molecular cloning methods
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cell cloning experiments
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