Sentence examples for molecular cloning techniques from inspiring English sources

All RNC constructs were prepared using standard molecular cloning techniques and cloned in the pBAD vector (Invitrogen) using the NcoI and HindIII restriction sites, resulting in an additional glycine after the initiator methionine.

More recently, combinatorial strategies for engineering AAV vectors using error-prone PCR, DNA shuffling, and other molecular cloning techniques have been established.

Since the first resilin sequence was identified in Drosophilia melanogaster (fruit fly), researchers have utilized molecular cloning techniques to construct resilin-based proteins for a number of different applications.

In recent years the availability of molecular cloning techniques allowed the isolation of the genes coding for the GtH subunits in 56 fish species representing at least 14 teleost orders.

Preimplantation genetic diagnosis (PGD) is utilizing modern molecular cloning techniques to detect genetic and chromosomal aberrations in early embryos originating from patients with inborn errors at risk for hereditary diseases or age-related risk for abnormal karyotype.

All molecular cloning techniques were carried out as described elsewhere (Sambrook and Russell 2001).

We can still certainly distinguish an identification process for a very new compound, which is almost unchanged since the very early days of allergen identification and today called 'proteomics', but at the same time several different approaches can be applied to homologous allergen identification by using molecular cloning technique [ 5].

To install fGly, a short consensus sequence, CXPXR where X is usually serine, threonine, alanine, or glycine is inserted at the desired location in the conserved regions of antibody heavy or light chains using standard molecular biology cloning techniques.

For plasmid constructions, standard molecular cloning strategies and techniques were applied (Sambrook et al., 1989).

The specific receptor involved with HDL cholesterol uptake was discovered to be the scavenger receptor class B type I (SR-BI) through molecular cloning and pharmacological techniques.

Practical applications of biotechnology and molecular bioengineering including recombinant DNA techniques, molecular cloning, microbial cell growth and manipulation, and library screening.