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All constructs were made using standard molecular cloning methods.
Using protein purification and molecular cloning methods, researchers have detected multiple xylanases in rumen bacteria and fungi [10], [11], [12].
It has been estimated by traditional molecular cloning methods that about 5% of genes in eukaryotic organisms experienced AS [3].
Molecular cloning methods, protein purification methods, primer sequences.
Plasmid FLAG-sPRDM16 was generated from MSCV-PRDM16 by standard molecular cloning methods.
General molecular cloning methods were performed as described in reference [ 19].
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Different concentrations of tripterine treated DU145 cells and control cells, with cell lysate 100 μL pre-cooling (configuration according to molecular cloning method) the ice cracking 30 min, extraction of total cellular protein, quantitative protein by Lowry method.
In animals, discovery of miRNA genes, by using molecular cloning based methods has been supplemented by systematic computational approaches that identify evolutionarily conserved miRNA genes.
However, to further accelerate progress and extend the repertoire of molecular tools, efficient cloning methods are needed.
A complete circumvention of molecular cloning steps qualifies this method for sequencing genome segments that are regarded unclonable, and thus unsequenceable by the traditional methods.
However, identification of the extent of genetic variation in a viral quasispecies under a given condition requires a highly accurate sequencing method that does not rely on molecular cloning, or a phenotypic selection method such as plaque generation.
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molecular imprinting methods
molecular biology methods
molecular cloning protocols
molecular cloning projects
molecular cloning techniques
molecular cloning reagents
molecular cloning experiments
molecular cloning studies
molecular simulation methods
molecular testing methods
molecular characterization methods
dna cloning methods
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