Sentence examples for modules were cloned from inspiring English sources

GST fusion proteins containing one to three HABD modules were cloned, expressed, and purified.

All modules were cloned each in pJET1.2/blunt using the CloneJET™ PCR Cloning Kit (Fermentas; Waltham, MA, USA) according to the instructions of the manufacturer.

Linker peptide modules were cloned according to Figure 1 (F,G) (see Materials and Methods), expressed in E. coli and purified.

Genes encoding cohesin modules were cloned using specific primers (Additional file 3: Table S1) by PCR from C. clariflavum genomic DNA using Reddymix x2 (Advanced Biotechnologies Ltd., Epsom, Surrey, United Kingdom).

Different numbers of this module were cloned between N and C-terminal capping repeats, i.e. ARs designed to shield the hydrophobic core of stacked AR modules.

SCR-modules were cloned via EcoRI and XbaI into pPICZ αA after modification of their flanking sequences introduced by PCR with the forward primers 5′-AA GAATTCGTAGCATCATGTGCACC-3′ and 5′-AA GAATTCTCAACAGGGAAATGTGG-3′ and reverse primers 5′-TAGATCTCTCCAGTTGGTCTG TCTAGAC-3′ and 5′-AA GAATTCTACACAAGTGGGATAATTG-3′ for SCR1112 and SCR1920, respectively (EcoRI and XbaI restriction sites are underlined).

In this context, each catalytic module was cloned as a unit together with its intact wild-type linker to which the desired component (CBM, dockerin or enzyme) was appended.

The modules obtained were cloned into the pRS416 plasmid, thus generating pRS416 PBand-KlBAT1 and pRS416 plasmidsBAT1 plasmids.

Recombinant cohesin and dockerin modules that were identified from the C. clariflavum genome were cloned into matching fusion-protein cassettes and expressed.

The three fragments were cloned into pET21a by Seamless Cloning.

Positive clones were cloned twice by limiting dilution.