Sentence examples for elements were cloned from inspiring English sources

To minimize interference between the different tetracycline-inducible promoters used in this study (tetracycline-responsive element (TRE), TRE-tight, or Tk-tetO) and the rtTA-M2 transactivator, both elements were cloned in opposite directions and separated by a 5 kb human p53 intron.

Potential regulatory elements were cloned into pGL2-Promoter (Promega) and transfected into MCF7 cells using the TransIT-LT1 Transfection Reagent (Mirus).

All elements were cloned into reporter vectors and assessed for expression modulation in dual luciferase reporter assays.

The ability of the γ-secretase-cleaved NICD to bind to CBF1 and activate gene transcription was measured by the transfection of a reporter in which four copies of the wild-type CBF1-binding elements were cloned in front of a simian virus 40 promoter-driven luciferase gene (4xwtCBF1Luc) (Hsieh et al, 1996).

Separate enhancer elements are cloned upstream of an hsp70 promoter.

All cis-elements were cloned into the pGL4.24 Luciferase reporter vector (Promega, Madison, USA).

The PCR products for the human, M. mulatta and C. aethiops Alu-elements were cloned into the pCR-2.1 Topo-TA vector (Invitrogen, Carlsbad, CA).

The bait element was cloned into the vector pONE-1 upstream of the Gal1,10 minimal promoter and the HIS3-cDNA.

A trimer of the CE1 element was cloned in front of the minimal promoters of the lacZ and the HIS3 reporter genes, respectively.

A galFF element was cloned in front of a 7 kb kdrl promoter fragment (Beis et al., 2005) within a miniTol2 vector.

Since the Sec residue of mammalian TrxR is close to the C-terminus, a SECIS element was cloned at the 3′-end of the mammalian gene (Arnér et al. 1999).