Sentence examples for samples were cloned from inspiring English sources

The amplicons of 57 out of 89 PCR-positive samples were cloned and sequenced.

PCR products from Treponema samples were cloned directly as described below.

To identify the corresponding HFE transcripts, the RT-PCR products from all samples were cloned and sequenced.

Isolated MC1R sequences from ancient DNA samples were cloned and transfected into cell lines to examine the function of the receptor in activation assays in vitro.

CDKN2B exon 1 PCR fragments from tumour samples were cloned and then sequenced.

PCR products of one to three S. schwerinii samples were cloned per gene segment.

PCR products from each sample were cloned onto pGEM-T Easy Vector (Promega, USA), followed by transformation into JM109 competent Escherichia coli cells (Promega).

PCR products from three separate PCRs for each sample were cloned using the pGEM-T Easy Vector System kit (Promega, Madison, WI, USA).

A p289-290 amplified amplified on a canine sample, was cloned into pGEMt-Easy (pSTD) (Promega, Madison, WI, USA) and sequenced at the GIGA facilities of the University of Liège.

PCR-amplified pfcrt DNA from 9 of these samples (no DNA was available for the 10th sample) was cloned into the TOPO TA vector, transfected into the TOP10 strain of E. coli, grown on selective medium, and sequenced.

However, because all LRIM1 and CEC1 samples that were cloned were also sequenced directly, we were able to derive both alleles from each individual while removing PCR/cloning errors.